Article
Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly.
Cell - 12 Feb 1993
Tollervey D, Lehtonen H, Jansen R, Kern H, Hurt E C
Abstract excerpt
We have generated temperature-sensitive lethal point mutations in the small nucleolar RNA-associated protein fibrillarin (encoded by the NOP1 gene in yeast) and analyzed their effects on ribosome synthesis. The five alleles tested all prevent synthesis of normal ribosomes, but in dramatically dif...
Topics
- Base Sequence
- Cell Nucleolus
- Chromosomal Proteins, Non-Histone
- Methylation
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- RNA Precursors
- RNA Processing, Post-Transcriptional
- RNA, Ribosomal
- Ribosomes
- Saccharomyces cerevisiae
- Temperature