Article
RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.
Molecular and cellular biology - 1 Jul 2000
Ossareh-Nazari B, Maison C, Black B E, Lévesque L, Paschal B M, Dargemont C
Abstract excerpt
To better characterize the mechanisms responsible for RNA export from the nucleus, we developed an in vitro assay based on the use of permeabilized HeLa cells. This new assay supports nuclear export of U1 snRNA, tRNA, and mRNA in an energy- and Xenopus extract-dependent manner. U1 snRNA export requires a 5' monomethylated cap structure, the nuclear export signal receptor CRM1, and the small GTPase Ran. In...
Topics
- Animals
- Biological Transport
- Carrier Proteins
- Female
- Guanosine Triphosphate
- Humans
- Hydrolysis
- Karyopherins
- Methylation
- Molecular Biology
- Mutation
- Nucleocytoplasmic Transport Proteins
- RNA
- RNA Caps
- RNA, Messenger