Article
Increase in ampC promoter strength due to mutations and deletion of the attenuator in a clinical isolate of cefoxitin-resistant Escherichia coli as determined by RT-PCR.
The Journal of antimicrobial chemotherapy - 1 May 2005
Tracz Dobryan M, Boyd David A, Bryden Louis, Hizon Romeo, Giercke Sandra, Van Caeseele Paul, Mulvey Michael R
Abstract excerpt
OBJECTIVES: To characterize the mechanism of cefoxitin resistance in clinical isolate Escherichia coli N99-0001. METHODS: Plasmid analysis, PCR for beta-lactamases, and sequencing of the ampC genes was carried out. An RT-PCR method was developed to determine relative ampC expression. RESULTS: Analysis of the ampC promoter region of E. coli N99-0001 revealed a T-->A mutation at -32, a C-->A mutation at -11, an...
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