Role of tryptophan 49 in the heparin cofactor activity of human antithrombin III.

Article

Role of tryptophan 49 in the heparin cofactor activity of human antithrombin III.

The Journal of biological chemistry - 25 Oct 1992

Gettins P, Choay J, Crews B C, Zettlmeiss G

Abstract excerpt

To probe the functional role of tryptophan 49 in human antithrombin III, a mutant antithrombin, W49K, has been expressed in baby hamster kidney cells. The mutation reduces the affinity for heparin pentasaccharide by 1.8 kcal mol-1 but does not alter the heparin enhancement of the rate of factor X...

Topics

Animals
Antithrombin III
Cells, Cultured
Cricetinae
Electrophoresis, Polyacrylamide Gel
Fluorescence
Heparin
Humans
Magnetic Resonance Spectroscopy
Mutation
Recombinant Proteins
Substrate Specificity
Tryptophan

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