Article
Elevation of sister chromatid exchange in Saccharomyces cerevisiae sgs1 disruptants and the relevance of the disruptants as a system to evaluate mutations in Bloom's syndrome gene.
Mutation research - 28 Apr 2000
Onoda F, Seki M, Miyajima A, Enomoto T
Abstract excerpt
The SGS1 of Saccharomyces cerevisiae is a homologue of the Bloom's syndrome and Werner's syndrome genes. The sgs1 disruptants show hyperrecombination, higher sensitivity to methyl methanesulfonate and hydroxyurea, and poor sporulation. In this study, we found that sister chromatid exchange was increased in sgs1 disruptants. We made mutated SGS1 genes coding a protein proved to lack DNA helicase activity...
Topics
- Alleles
- Bloom Syndrome
- DNA Helicases
- Dose-Response Relationship, Drug
- Genetic Complementation Test
- Hydroxyurea
- Methyl Methanesulfonate
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation, Missense
- Phenotype
- Plasmids
- RecQ Helicases