Article
Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry.
Nature protocols - 1 Jan 2008
Dong Hiep Phuc, Kleinberg Lilach, Davidson Ben, Risberg Björn
Abstract excerpt
We describe here a protocol for the detection of epithelial cells in effusions combined with quantification of apoptosis by flow cytometry (FCM). The procedure described consists of the following stages: culturing and induction of apoptosis by staurosporine in control ovarian carcinoma cell lines (SKOV-3 and OVCAR-8); preparation of effusion specimens and cell lines for staining; staining of cancer cells in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
