Article
A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance.
Yeast (Chichester, England) - 1 Apr 2000
Toyn J H, Gunyuzlu P L, White W H, Thompson L A, Hollis G F
Abstract excerpt
The ability to counterselect, as well as to select for, a genetic marker has numerous applications in microbial genetics. Described here is the use of 5-fluoroanthranilic acid for the counterselection of TRP1, a commonly used genetic marker in the yeast Saccharomyces cerevisiae. Counterselection using 5-fluoroanthranilic acid involves antimetabolism by the enzymes of the tryptophan biosynthetic pathway, such that...
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