Article
Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warning.
FEMS yeast research - 1 Feb 2008
González Asier, Larroy Carolina, Biosca Josep A, Ariño Joaquín
Abstract excerpt
The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses.
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