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Article

Amino acid 305 determines catalytic center accessibility in CYP3A4.

Biochemistry - 18 Apr 2000

Fowler S M, Riley R J, Pritchard M P, Sutcliffe M J, Friedberg T, Wolf C R

Abstract excerpt

Site-directed mutagenesis has been used to replace alanine 305 with phenylalanine (A305F) and serine (A305S) in the active site of cytochrome P450 3A4 (CYP3A4). Enzyme kinetics for diazepam, erythromycin, nifedipine, and testosterone metabolism have been determined for both mutants and wild-type CYP3A4. The A305F mutation abolished diazepam oxidase activity and reduced the S(50) and V(max) for erythromycin...

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Identifiers

PMID: 10757990
DOI: 10.1021/bi992372u

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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