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Article

Role of residue 478 as a determinant of the substrate specificity of cytochrome P450 2B1.

Biochemistry - 29 Sept 1992

He Y A, Balfour C A, Kedzie K M, Halpert J R

Abstract excerpt

Two allelic variants and eight site-directed mutants of cytochrome P450 2B1 differing at residue 478 have been expressed in COS cells and assayed for androstenedione hydroxylase activities. The 478Gly and 478Ala variants and five mutants (Ser, Thr, Val, Ile, and Leu) exhibited 16 beta-OH:16 alpha-OH ratios ranging from 0.7 to 9.3, whereas the Pro, Glu, and Arg mutants were expressed but inactive. The seven...

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Identifiers

PMID: 1390709
DOI: 10.1021/bi00153a015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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