Article
Detection of genes encoding aminoglycoside-modifying enzymes in staphylococci by polymerase chain reaction and dot blot hybridization.
International journal of antimicrobial agents - 1 Feb 2000
Udo E E, Dashti A A
Abstract excerpt
Dot blot hybridization and the polymerase chain reaction (PCR) were used to study aminoglycoside-modifying enzymes in aminoglycoside-resistant staphylococci isolated in hospitals in Kuwait. DNA encoding the acetyltransferase (AAC) (6')-phosphotransferase (APH) (2"), nucleotidyltransferase (ANT) (4') and APH (3') enzymes were detected in Staphylococcus aureus and coagulase negative staphylococci. ANT (4') was the...
Topics
- Acetyltransferases
- Aminoglycosides
- Anti-Bacterial Agents
- Cross Infection
- Drug Resistance, Microbial
- Genotype
- Humans
- Kanamycin Kinase
- Microbial Sensitivity Tests
- Nucleic Acid Hybridization
- Nucleotidyltransferases
