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Article

Yeast cell-free system that catalyses joint-molecule formation in a Rad51p- and Rad52p-dependent fashion.

The Biochemical journal - 15 Apr 2000

Nagaraj V, Norris D

Abstract excerpt

One of the central reactions of homologous recombination is the invasion of a single strand of DNA into a homologous duplex to form a joint molecule. Here we describe the isolation of a cell-free system from meiotic yeast cells that catalyses joint-molecule formation in vitro. The active components in the system required ATP and homologous DNA and operated in both 0.5 and 13 mM MgCl(2). When the cell-free system...

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Identifiers

PMID: 10749664
DOI: 10.1042/0264-6021:3470363
PMCID: PMC1220967

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