Article
Analysis of the role of the hypervariable region of yeast Ras2p and its farnesylation in the interaction with exchange factors and adenylyl cyclase.
The Journal of biological chemistry - 9 Jun 2000
Créchet J B, Jacquet E, Bernardi A, Parmeggiani A
Abstract excerpt
Ras proteins from Saccharomyces cerevisiae differ from mammalian Ha-Ras in their extended C-terminal hypervariable region. We have analyzed the function of this region and the effect of its farnesylation with respect to the action of the GDP/GTP exchange factors (GEFs) Cdc25p and Sdc25p and the target adenylyl cyclase. Whereas Ras2p farnesylation had no effect on the interaction with purified GEFs from the Cdc25...
Topics
- Adenylyl Cyclases
- Catalytic Domain
- Cell Cycle Proteins
- Cell Membrane
- Fungal Proteins
- GTP-Binding Proteins
- Genetic Variation
- Guanosine Diphosphate
- Guanosine Triphosphate
- Kinetics
- Protein Prenylation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- ras Proteins
- ras-GRF1