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Article

In vitro reconstitution of cdc25 regulated S. cerevisiae adenylyl cyclase and its kinetic properties.

The EMBO journal - 1 Mar 1990

Engelberg D, Simchen G, Levitzki A

Abstract excerpt

The attenuated GTP regulation adenylyl cyclase (CDC35) lysates or membranes prepared from cells of a cdc25ts strain is enhanced 2.5- to 6-fold by mixing these lysates or membranes with lysates or membranes from a cdc35ts strain harboring wild-type CDC25. The kinetics of activation of the Saccharomyces cerevisiae adenylyl cyclase in vitro is first order, as is the activation of mammalian adenylyl cyclase. The rate...

Topics

Adenylyl Cyclases
Cell Cycle Proteins
Cell Membrane
Cyclic AMP
Enzyme Activation
Fungal Proteins
Genes, Fungal
Genotype
Guanylyl Imidodiphosphate
Kinetics
Plasmids
Saccharomyces cerevisiae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae Proteins

ras Proteins

ras-GRF1

Source

Open in PubMed Open full text

Identifiers

PMID: 2155776
DOI: 10.1002/j.1460-2075.1990.tb08156.x
PMCID: PMC551717

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2155776-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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In vitro reconstitution of cdc25 regulated S. cerevisiae adenylyl cyclase and its kinetic properties.PMID 2155776Read ↗

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