Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A rapid phenotypic assay for detection of acyclovir-resistant varicella-zoster virus with mutations in the thymidine kinase open reading frame.

Antimicrobial agents and chemotherapy - 1 Apr 2000

Sahli R, Andrei G, Estrade C, Snoeck R, Meylan P R

Abstract excerpt

Susceptibility assays by cell culture methods are time-consuming and are particularly difficult to perform with varicella-zoster virus (VZV). To overcome this limitation, we have adapted a functional test of the viral thymidine kinase (TK) in TK-deficient (tdk mutant) bacteria to detect ACV-resistant VZV in clinical samples. After PCR amplification, the complete viral TK open reading frame (ORF) is purified from...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Acyclovir
Share to Antiviral Agents

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Chickenpox

Share to Cloning, Molecular

Share to Herpesvirus 3, Human

Share to Open Reading Frames

Share to Reverse Transcriptase Polymerase Chain Reaction

Share to Thymidine Kinase

Source

Open in PubMed Open full text

Identifiers

PMID: 10722484
DOI: 10.1128/AAC.44.4.873-878.2000
PMCID: PMC89785

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A rapid phenotypic assay for detection of acyclovir-resistant varicella-zoster virus with mutations in the thymidine kinase open reading frame.PMID 10722484Read ↗

Phenotypic and genetic characterization of thymidine kinase from clinical strains of varicella-zoster virus resistant to acyclovir.Semantic proximity Read ↗

Drug Resistance of Clinical Varicella-Zoster Virus Strains Confirmed by Recombinant Thymidine Kinase Expression and by Targeted Resistance Mutagenesis of a Cloned Wild-Type IsolateSemantic proximity Read ↗

Complementary assays for monitoring susceptibility of varicella-zoster virus resistance to antivirals.Semantic proximity Read ↗

Random mutagenesis of the thymidine kinase gene of varicella-zoster virus.Semantic proximity Read ↗

Analysis of the thymidine kinase genes from acyclovir-resistant mutants of varicella-zoster virus isolated from patients with AIDS.Semantic proximity Read ↗

Phenotypic and genetic characterization of varicella-zoster virus mutants resistant to acyclovir, brivudine and/or foscarnet.Semantic proximity Read ↗

Genetic polymorphism of thymidine kinase (TK) and DNA polymerase (pol) of clinical varicella-zoster virus (VZV) isolates collected over three decades.Semantic proximity Read ↗

Phenotypic testing of patient herpes simplex virus type 1 and 2 isolates for acyclovir resistance by a novel method based on real-time cell analysis.Semantic proximity Read ↗

Acyclovir-resistant varicella-zoster virus: phenotypic and genetic characterization.Semantic proximity Read ↗

Analysis of mutations in the thymidine kinase genes of drug-resistant varicella-zoster virus populations using the polymerase chain reaction.Semantic proximity Read ↗

Role of genotypic analysis of the thymidine kinase gene of herpes simplex virus for determination of neurovirulence and resistance to acyclovir.Semantic proximity Read ↗

Phenotypic and genotypic characterization of clinical isolates of herpes simplex virus resistant to aciclovir.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.