Article
A C-terminal truncation results in high-level expression of the functional photoreceptor sensory rhodopsin I in the archaeon Halobacterium salinarium.
Molecular microbiology - 1 Sept 1993
Ferrando-May E, Brustmann B, Oesterhelt D
Abstract excerpt
Expression of the gene encoding the halobacterial photoreceptor sensory rhodopsin I (SRI), sopI, was studied by means of homologous gene targeting. A sopI- Halobacterium salinarium mutant strain was constructed by homologous replacement of sopI with a novobiocin-resistant gyrB from Haloferax Aa 2...
Topics
- Amino Acid Sequence
- Bacteriorhodopsins
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Cell Membrane
- DNA, Bacterial
- Drug Resistance, Microbial
- Gene Expression
- Genes, Bacterial
- Genetic Complementation Test
- Genetic Vectors
- Halobacteriaceae
- Halobacterium
- Halorhodopsins
- Molecular Sequence Data
- Mutagenesis, Site-Directed