Article
A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris.
Gene - 17 Aug 1998
Shen S, Sulter G, Jeffries T W, Cregg J M
Abstract excerpt
In methylotrophic yeasts, glutathione-dependent formaldehyde dehydrogenase (FLD) is a key enzyme required for the metabolism of methanol as a carbon source and certain alkylated amines such as methylamine as nitrogen sources. We describe the isolation and characterization of the FLD1 gene from th...
Topics
- Alcohol Oxidoreductases
- Aldehyde Oxidoreductases
- Amino Acid Sequence
- Base Sequence
- DNA, Fungal
- Enzyme Stability
- Gene Expression Regulation, Fungal
- Molecular Sequence Data
- Mutation
- Nitrogen Compounds
- Pichia
- Promoter Regions, Genetic
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Temperature
- Transgenes