Article
Quantification of illegitimate V(D)J recombinase-mediated mutations in lymphocytes of newborns and adults.
Mutation research - 17 Dec 1999
Scheerer J B, Xi L, Knapp G W, Setzer R W, Bigbee W L, Fuscoe J C
Abstract excerpt
We used a direct polymerase chain reaction (PCR) method for quantification of HPRT exons 2 + 3 deletions and t(14;18) translocations as a measure of illegitimate V(D)J recombination. We determined the baseline frequencies of these two mutations in mononuclear leukocyte DNA from the umbilical cord blood of newborns and from the peripheral blood of adults. In an initial group of 21 newborns, no t(14;18)...
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