Article
Intermediates of rifamycin polyketide synthase produced by an Amycolatopsis mediterranei mutant with inactivated rifF gene.
Microbiology (Reading, England) - 1 Dec 1999
Stratmann Ansgar, Toupet Christiane, Schilling Wolfgang, Traber René, Oberer Lukas, Schupp Thomas
Abstract excerpt
Rifamycin B biosynthesis in Amycolatopsis mediterranei N/813 was inactivated by introducing a small deletion in the rifF gene situated directly downstream of the rifamycin polyketide synthase (PKS) gene cluster. The corresponding mutant strain produced a series of linear intermediates of rifamycin B biosynthesis that are most probably generated by obstruction of the normal release of the end product of the...
Topics
- Actinomycetales
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Chromatography, High Pressure Liquid
- Electroporation
- Gene Deletion
- Genetic Engineering
- Molecular Sequence Data
- Multienzyme Complexes