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Article

Cloning and preliminary characterization of lh3 gene encoding a putative acetyltransferase from a rifamycin SV-producing strain Amycolatopsis mediterranei.

Biotechnology letters - 1 Aug 2005

Chen Lei, Yu Hao, Lu Yinhua, Jiang Weihong

Abstract excerpt

An ORF located immediately downstream of glnR gene was cloned from Amycolatopsis mediterranei U32 and was named lh3. Sequence analysis revealed that lh3 encodes a putative acetyltransferase, which shows high amino acid sequence similarities to the mycothiol synthase (MshD) from other actinomycetes. For functional analysis, mutation in lh3 gene was generated by gene replacement with an apramycin resistance gene...

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PMID: 16132864
DOI: 10.1007/s10529-005-8462-z

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