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Article

Identification of beta-secretase-like activity using a mass spectrometry-based assay system.

Nature biotechnology - 1 Jan 2000

Grüninger-Leitch F, Berndt P, Langen H, Nelboeck P, Döbeli H

Abstract excerpt

We describe an assay system for the identification of site-specific proteases. The assay is based on a protein substrate that is immobilized on ceramic beads. After incubation with cell homogenates, the beads are washed and digested with endoproteinase Lys-C to liberate a defined set of peptides. The peptide fragments are identified by mass spectrometry. The assay was used to screen for beta-secretase, the...

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Source

Identifiers

PMID: 10625394
DOI: 10.1038/71944

Genes

APP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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