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Article

Structure-function analysis of human cytochrome P450 3A4 using 7-alkoxycoumarins as active-site probes.

Archives of biochemistry and biophysics - 15 Jan 2000

Khan K K, Halpert J R

Abstract excerpt

The oxidation of a series of seven alkyl ethers of 7-hydroxycoumarin by cytochrome P450 3A4 (CYP3A4) has been studied to probe the active site of the enzyme. TLC of the reaction mixture showed formation of metabolites other than 7-hydroxycoumarin. The separation and characterization of the different metabolites of the C4 to C7 compounds were achieved using a combination of TLC, HPLC, and gas...

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Source

Identifiers

PMID: 10620357
DOI: 10.1006/abbi.1999.1578

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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