Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Engineering of human CYP3A enzymes by combination of activating polymorphic variants.

Applied biochemistry and biotechnology - 1 Oct 2012

Neunzig Ina, Widjaja Maria, Drăgan Călin-Aurel, Peters Frank T, Maurer Hans H, Bureik Matthias

Abstract excerpt

The use of human cytochrome P450 (CYP) enzymes is increasing for the production of drug metabolites used for drug safety testing and doping analysis. Major challenges are high-priced cofactors, poor stability, and comparatively low activities. We have shown previously that production of specific metabolites in milligrams to gram scale is feasible using human CYPs recombinantly expressed in fission yeast. In this...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Biotransformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cytochrome P-450 CYP3A

Share to Enzyme Activation

Share to Plasmids

Share to Polymorphism, Genetic

Share to Protein Engineering

Share to Schizosaccharomyces

Source

Identifiers

PMID: 22899011
DOI: 10.1007/s12010-012-9819-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Engineering of human CYP3A enzymes by combination of activating polymorphic variants.PMID 22899011Read ↗

Molecular insights into CYP19A1 mutations and their role in estrogen production.Semantic proximity Read ↗

Altered human CYP3A4 activity caused by Antley-Bixler syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system.Semantic proximity Read ↗

Loss of multiple enzyme activities due to the human genetic variation P284T in NADPH cytochrome P450 oxidoreductaseSemantic proximity Read ↗

Functional characterization of eight human CYP1A2 variants: the role of cytochrome b5.Semantic proximity Read ↗

Protein engineering of human cytochromes P450 and their allelic variants for nanobiotechnological applicationsSemantic proximity Read ↗

Enhancement of 7-methoxyresorufin O-demethylation activity of human cytochrome P450 1A2 by molecular breeding.Semantic proximity Read ↗

Reduction in hepatic drug metabolizing CYP3A4 activities caused by P450 oxidoreductase mutations identified in patients with disordered steroid metabolism.Semantic proximity Read ↗

Allosteric Activation of Cytochrome P450 3A4 via Progesterone Bioconjugation.Semantic proximity Read ↗

Structure-function analysis of human cytochrome P450 3A4 using 7-alkoxycoumarins as active-site probes.Semantic proximity Read ↗

Exploring the potential of xenobiotic-metabolising enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes.Semantic proximity Read ↗

Impacts of amino acid substitutions in fungal cytochrome P450 monooxygenase (CYP57B3) on the effective production of 3΄-hydroxygenistein.Semantic proximity Read ↗

Heterotropic and homotropic cooperativity by a drug-metabolising mutant of cytochrome P450 BM3.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.