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Article

Stoichiometry of 7-ethoxycoumarin metabolism by cytochrome P450 2B1 wild-type and five active-site mutants.

FEBS letters - 13 Oct 1997

Fang X, Kobayashi Y, Halpert J R

Abstract excerpt

Recombinant P450 2B1 wild-type and the active-site mutants I114V, F206L, V363A, V363L, and G478S were purified and studied. The efficiency of coupling of reducing equivalents to 7-hydroxycoumarin formation was decreased for all the mutants except I114V. Uncoupling to H2O was increased for F206L,...

Topics

Binding Sites
Coumarins
Cytochrome P-450 CYP2B1
Hydrogen Peroxide
Methylation
Mutation
NADP
Recombinant Proteins
Water

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 9369237
DOI: 10.1016/s0014-5793(97)01173-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9369237-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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