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Article

Medium perfusion enhances osteogenesis by murine osteosarcoma cells in three-dimensional collagen sponges.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Dec 1999

Mueller S M, Mizuno S, Gerstenfeld L C, Glowacki J

Abstract excerpt

In this study, we examined in vitro histogenesis by murine K8 osteosarcoma cells maintained in three-dimensional (3D) collagen sponges. We tested the hypothesis that perfusion of medium enhances cell viability and their biosynthetic activity as assessed by expression of the osteoblastic phenotype and mineral deposition. At intervals, samples were harvested and analyzed histologically, biochemically, and by...

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PMID: 10620071
DOI: 10.1359/jbmr.1999.14.12.2118

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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