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Article

Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase.

Genes & development - 1 Dec 1999

Kim D R, Dai Y, Mundy C L, Yang W, Oettinger M A

Abstract excerpt

The RAG1 and RAG2 proteins collaborate to initiate V(D)J recombination by binding recombination signal sequences (RSSs) and making a double-strand break between the RSS and adjacent coding DNA. Like the reactions of their biochemical cousins, the bacterial transposases and retroviral integrases, cleavage by the RAG proteins requires a divalent metal ion but does not involve a covalent protein/DNA intermediate. In...

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Source

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Identifiers

PMID: 10601033
DOI: 10.1101/gad.13.23.3070
PMCID: PMC317176

Genes

RAG1
RAG2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutations of acidic residues in RAG1 define the active site of the V(D)J recombinase.PMID 10601033Read ↗

Mutational analysis of all conserved basic amino acids in RAG-1 reveals catalytic, step arrest, and joining-deficient mutants in the V(D)J recombinase.Semantic proximity Read ↗

Rag-1 mutations associated with B-cell-negative scid dissociate the nicking and transesterification steps of V(D)J recombination.Semantic proximity Read ↗

A zinc site in the C-terminal domain of RAG1 is essential for DNA cleavage activity.Semantic proximity Read ↗

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Semantic proximity Read ↗

The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination.Semantic proximity Read ↗

RAG proteins shepherd double-strand breaks to a specific pathway, suppressing error-prone repair, but RAG nicking initiates homologous recombination.Semantic proximity Read ↗

Requirements for DNA hairpin formation by RAG1/2.Semantic proximity Read ↗

Crystal structure of the V(D)J recombinase RAG1-RAG2.Semantic proximity Read ↗

Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination.Semantic proximity Read ↗

Human RAG mutations: biochemistry and clinical implications.Semantic proximity Read ↗

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.Semantic proximity Read ↗

Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.Semantic proximity Read ↗

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