Article
Analysis of E-cadherin in diffuse-type gastric cancer using a mutation-specific monoclonal antibody.
The American journal of pathology - 1 Dec 1999
Becker K F, Kremmer E, Eulitz M, Becker I, Handschuh G, Schuhmacher C, Müller W, Gabbert H E, Ochiai A, Hirohashi S, Höfler H
Abstract excerpt
In-frame deletions from the E-cadherin mRNA, coding for a homophilic cell adhesion molecule, are characteristic for diffuse-type gastric carcinomas. Using immunohistochemical analysis the mutant form cannot be distinguished from normal E-cadherin, making results difficult to interpret. In this study, a rat monoclonal antibody, designated E-cad delta 9-1, was generated against a peptide spanning the fusion...
Topics
- Animals
- Antibodies, Monoclonal
- Biomarkers, Tumor
- Blotting, Western
- Cadherins
- Epitopes
- Humans
- Immunohistochemistry
- Mutation
- RNA, Messenger
- Rats
- Stomach Neoplasms
- Transfection
- Tumor Cells, Cultured