Article
Interdomain signaling in glutamine phosphoribosylpyrophosphate amidotransferase.
The Journal of biological chemistry - 17 Dec 1999
Bera A K, Chen S, Smith J L, Zalkin H
Abstract excerpt
The glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase-catalyzed synthesis of phosphoribosylamine from PRPP and glutamine is the sum of two half-reactions at separated catalytic sites in different domains. Binding of PRPP to a C-terminal phosphoribosyltransferase domain is required to activate the reaction at the N-terminal glutaminase domain. Interdomain signaling was monitored by intrinsic tryptophan...
Topics
- Amidophosphoribosyltransferase
- Bacterial Proteins
- Binding Sites
- Enzyme Activation
- Escherichia coli
- Mutation
- Phosphoribosyl Pyrophosphate
- Protein Binding
- Structure-Activity Relationship
- Substrate Specificity