Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type amidotransferases.

Abstract excerpt

A series of deletions was constructed in cloned Escherichia coli purF encoding glutamine phosphoribosylpyrophosphate amidotransferase. These deletions extended into the NH2 terminus of the protein and removed amino acids that are required for glutamine-dependent enzyme activity. Enzyme function,...

Topics

• Amidophosphoribosyltransferase
• Chromosome Deletion
• Escherichia coli
• Glutamine
• Mutation
• Pentosyltransferases
• Structure-Activity Relationship