Use of a PCR-based assay for fetal Cw antigen genotyping in a patient with a history of moderately severe hemolytic disease of the newborn due to anti-Cw.

Abstract excerpt

Anti-Cw is an uncommon cause of clinically significant hemolytic disease of the newborn (HDN). We report an unusually severe case of HDN due to anti-Cw that required phototherapy and exchange transfusion. We also describe a novel PCR-RFLP method for Cw typing of fetal genomic DNA that was used for prenatal diagnosis in a subsequent pregnancy. Following PCR amplification of a 163 bp segment of the RHCE gene...

Topics

• Adult
• Erythroblastosis, Fetal
• Female
• Genotype
• Humans
• Infant, Newborn
• Isoantibodies
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Pregnancy
• Prenatal Diagnosis
• Rh-Hr Blood-Group System