Article
Oligonucleotide-based inhibition of embryonic gene expression.
Nature biotechnology - 1 Dec 1999
Driver S E, Robinson G S, Flanagan J, Shen W, Smith L E, Thomas D W, Roberts P C
Abstract excerpt
We describe a technique to define gene function using antisense oligonucleotide (AS-ODN) inhibition of gene expression in mice. A single intravenous injection of an AS-ODN targeting vascular endothelial growth factor (VEGF) into pregnant mice between E7.5-8.5 resulted in a lack of primary angiogenesis. This enabled us to define the critical window required to inhibit VEGF expression and recapitulate the primary...
Topics
- Animals
- Base Sequence
- Cadherins
- Dose-Response Relationship, Drug
- Endothelial Growth Factors
- Female
- Gene Expression Regulation, Developmental
- Lymphokines
- Mice
- Mice, Knockout
- Oligonucleotides, Antisense
- Phenotype
- Pregnancy
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors