Article
Detection of simple mutations and polymorphisms in large genomic regions.
Nucleic acids research - 15 Nov 2001
Sokurenko E V, Tchesnokova V, Yeung A T, Oleykowski C A, Trintchina E, Hughes K T, Rashid R A, Brint J M, Moseley S L, Lory S
Abstract excerpt
We have developed a novel technology that makes it possible to detect simple nucleotide polymorphisms directly within a sample of total genomic DNA. It allows, in a single Southern blot experiment, the determination of sequence identity of genomic regions with a combined length of hundreds of kilobases. This technology does not require PCR amplification of the target DNA regions, but exploits preparative...
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