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Article

Recombination of the GFP gene to the BFP gene using a man-made site-selective DNA cutter.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry - 1 Jan 2006

Kitamura Yoshihito, Mori Satoshi, Chen Wen, Sumaoka Jun, Komiyama Makoto

Abstract excerpt

By using the recently developed man-made DNA cutter [a combination of Ce(IV)/EDTA and two DNA additives], green fluorescent protein (GFP) was converted to closely related blue fluorescent protein (BFP). The phosphodiester linkages at T196-A200 in the sense strand of GFP were hydrolyzed by the cutter, and the A1-T196 fragment in the product was selectively connected with the downstream fragment (C197-A720) of BFP...

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Identifiers

PMID: 16341899
DOI: 10.1007/s00775-005-0063-8

Genes

CAT
TAT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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