Article
Alanine-23 of transducin alpha subunit is involved in defining the affinity for betagamma complex.
Neuroreport - 23 Jun 1999
Ho M K, Wong Y H
Abstract excerpt
Transducin a subunit (alpha(t1)) shows extraordinarily high affinity to G protein beta(gamma) complex. One of the beta(gamma)-binding regions on alpha(t1) is the amino-terminal helix. Alanine-23 is uniquely found on alpha(t1) but not other members of the G(i)-subfamily. Mutation of alanine-23 into serine reduced the ability of alpha(t1) to sequester beta(gamma)-mediated stimulation of type II adenylyl cyclase....
Topics
- Alanine
- Analgesics
- Antisense Elements (Genetics)
- Cells, Cultured
- Cyclic AMP
- Enkephalins
- GTP-Binding Proteins
- Humans
- Kidney
- Models, Molecular
- Mutagenesis
- Phenotype