Article
Two discontinuous segments in the carboxyl terminus are required for membrane targeting of the rat gamma-aminobutyric acid transporter-1 (GAT1).
The Journal of biological chemistry - 2 Jul 2004
Farhan Hesso, Korkhov Vladimir M, Paulitschke Verena, Dorostkar Mario M, Scholze Petra, Kudlacek Oliver, Freissmuth Michael, Sitte Harald H
Abstract excerpt
Like all members of the Na(+)/Cl(-)-dependent neurotransmitter transporter family, the rat gamma-aminobutyric acid transporter-1 (GAT1) is sorted and targeted to specialized domains of the cell surface. Here we identify two discontinuous signals in the carboxyl terminus of GAT1 that cooperate to drive surface expression. This conclusion is based on the following observations. Upon deletion of the last 37 amino...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Amino Acids
- Animals
- Bacterial Proteins
- Biotinylation
- Carrier Proteins
- Cell Differentiation
- Cell Line
- Cell Membrane