Article
Resistance of butyrylcholinesterase to inactivation by ultrasound: effects of ultrasound on catalytic activity and subunit association.
Biochimica et biophysica acta - 8 Sept 1998
Froment M T, Lockridge O, Masson P
Abstract excerpt
The effects of 20 kHz ultrasound on catalytic activity and structure of the tetramer of wild-type human butyrylcholinesterase (BChE) from plasma and recombinant D70G mutant enzyme were studied at constant temperature. Effects on catalytic properties of both enzymes were investigated by kinetic an...
Topics
- Aspirin
- Binding Sites
- Butyrylcholinesterase
- Butyrylthiocholine
- Electrophoresis, Polyacrylamide Gel
- Humans
- Kinetics
- Models, Molecular
- Mutation
- Phenylbutyrates
- Protein Conformation
- Protein Denaturation
- Recombinant Proteins
- Ultrasonics