Article
Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation.
Clinical chemistry - 1 Sept 1996
Pastinen T, Partanen J, Syvänen A C
Abstract excerpt
We developed a multiplex, solid-phase minisequencing method to detect multiple single-nucleotide polymorphisms in an undivided sample. The amplified DNA templates are first captured on a manifold. Then, with multiple minisequencing primers of various sizes, single-nucleotide extension reactions a...
Topics
- Alleles
- Base Sequence
- Cell Line
- DNA Primers
- Fluorescence
- Gene Amplification
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- HLA-DR2 Antigen
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sequence Analysis, DNA
- Templates, Genetic