Article
Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation.
Clinical chemistry - 1 Sept 1996
Pastinen T, Partanen J, Syvänen A C
Abstract excerpt
We developed a multiplex, solid-phase minisequencing method to detect multiple single-nucleotide polymorphisms in an undivided sample. The amplified DNA templates are first captured on a manifold. Then, with multiple minisequencing primers of various sizes, single-nucleotide extension reactions are carried out simultaneously with fluorescently labeled dideoxynucleotides. The size of the extended product,...
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