Article
Integration of multiple PCR amplifications and DNA mutation analyses by using oligonucleotide microchip.
Analytical biochemistry - 1 May 2001
Tillib S V, Strizhkov B N, Mirzabekov A D
Abstract excerpt
We have developed a method for parallel independent on-chip amplification and the following sequence variation analysis of multiple DNA regions directly using microchip with an array of nanoliter gel pads containing specific sets of tethered primers. The method has three key features. First, DNA to be amplified is enriched at gel pads by its hybridization with immobilized primers. Second, different sets of...
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