Article
Modulation of gene expression in Leishmania drug resistant mutants as determined by targeted DNA microarrays.
Nucleic acids research - 15 Oct 2003
Guimond Chantal, Trudel Nathalie, Brochu Christian, Marquis Nathalie, El Fadili Amal, Peytavi Régis, Briand Guylaine, Richard Dave, Messier Nadine, Papadopoulou Barbara, Corbeil Jacques, Bergeron Michel G, Légaré Danielle, Ouellette Marc
Abstract excerpt
In the protozoan parasite Leishmania, drug resistance can be a complex phenomenon. Several metabolic pathways and membrane transporters are implicated in the resistance phenotype. To monitor the expression of these genes, we generated custom DNA microarrays with PCR fragments corresponding to 44 genes involved with drug resistance. Transcript profiling of arsenite and antimony resistant mutants with these arrays...
Topics
- Animals
- Antimony
- Arsenites
- Blotting, Northern
- Drug Resistance, Multiple
- Folic Acid Antagonists
- Gene Expression Profiling
- Gene Expression Regulation
- Genes, Protozoan