Article
Identification of residues involved in v-Src substrate recognition by site-directed mutagenesis.
FEBS letters - 13 Aug 1999
Yokoyama N, Miller W T
Abstract excerpt
To study the role of the catalytic domain in v-Src substrate specificity, we engineered three site-directed mutants (Leu-472 to Tyr or Trp and Thr-429 to Met). The mutant forms of Src were expressed in Sf9 cells and purified. We analyzed the substrate specificities of wild-type v-Src and the mutants using two series of peptides that varied at residues C-terminal to tyrosine. The peptides contained either the YMTM...
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