Article
Amino acid residues involved in the interaction of acetylcholinesterase and butyrylcholinesterase with the carbamates Ro 02-0683 and bambuterol, and with terbutaline.
Biochimica et biophysica acta - 17 Aug 1999
Kovarik Z, Radić Z, Grgas B, Skrinjarić-Spoljar M, Reiner E, Simeon-Rudolf V
Abstract excerpt
In order to identify amino acids involved in the interaction of acetylcholinesterase (AChE; EC 3.1.1.7) and butyrylcholinesterase (BChE; EC 3.1.1.8) with carbamates, the time course of inhibition of the recombinant mouse enzymes BChE wild-type (w.t.), AChE w.t. and of 11 site-directed AChE mutants by Ro 02-0683 and bambuterol was studied. In addition, the reversible inhibition of cholinesterases by terbutaline,...
Topics
- Acetylcholinesterase
- Amino Acids
- Animals
- Binding Sites
- Butyrylcholinesterase
- Carbamates
- Cholinesterase Inhibitors
- Humans
- Isoleucine
- Kinetics
- Mice
- Models, Molecular
- Mutagenesis, Site-Directed