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Article

Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.

RNA (New York, N.Y.) - 1 Jul 2009

Kershaw Christopher J, Barrass J David, Beggs Jean D, O'Keefe Raymond T

Abstract excerpt

The U5 snRNA loop 1 aligns the 5' and 3' exons for ligation during the second step of pre-mRNA splicing. U5 is intimately associated with Prp8, which mediates pre-mRNA repositioning within the catalytic core of the spliceosome and interacts directly with U5 loop 1. The genome-wide effect of three U5 loop 1 mutants has been assessed by microarray analysis. These mutants exhibited impaired and improved splicing of...

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Identifiers

PMID: 19447917
DOI: 10.1261/rna.1347409
PMCID: PMC2704078

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Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.PMID 19447917Read ↗

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