Article
Cloning, sequencing, expression and allelic sequence diversity of ERG3 (C-5 sterol desaturase gene) in Candida albicans.
Gene - 5 Aug 1999
Miyazaki Y, Geber A, Miyazaki H, Falconer D, Parkinson T, Hitchcock C, Grimberg B, Nyswaner K, Bennett J E
Abstract excerpt
The C-5 sterol desaturase gene (ERG3), essential for yeast ergosterol biosynthesis, was cloned and sequenced from Candida albicans by homology with the Saccharomyces cerevisiae ERG3. The ERG3 ORF contained 1158bp and encoded 386 deduced amino acids. The clone was used to transform a gal1 mutant derived from the Darlington strain of C. albicans, using galactose selection. The Darlington strain is known to lack...
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