Article
Isolation and characterization of an Arabidopsis thaliana cDNA encoding a delta 7-sterol-C-5-desaturase by functional complementation of a defective yeast mutant.
The Plant journal : for cell and molecular biology - 1 Mar 1996
Gachotte D, Husselstein T, Bard M, Lacroute F, Benveniste P
Abstract excerpt
A yeast null mutant (erg 3) defective in ERG 3, the gene encoding the C-5 sterol desaturase required for ergosterol synthesis was transformed with an Arabidopsis thaliana cDNA library inserted in a yeast vector. Transformants (4 x 10(5)) were screened for cycloheximide (CH) resistance and 400 possible clones were analyzed to determine their sterol profile. Low levels of ergosterol in addition to delta 7- and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.