Article
Isolation and characterization of an Arabidopsis thaliana cDNA encoding a delta 7-sterol-C-5-desaturase by functional complementation of a defective yeast mutant.
The Plant journal : for cell and molecular biology - 1 Mar 1996
Gachotte D, Husselstein T, Bard M, Lacroute F, Benveniste P
Abstract excerpt
A yeast null mutant (erg 3) defective in ERG 3, the gene encoding the C-5 sterol desaturase required for ergosterol synthesis was transformed with an Arabidopsis thaliana cDNA library inserted in a yeast vector. Transformants (4 x 10(5)) were screened for cycloheximide (CH) resistance and 400 pos...
Topics
- Amino Acid Sequence
- Arabidopsis
- Base Sequence
- DNA, Complementary
- DNA, Plant
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Oxidoreductases
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid