Article
Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation.
Proceedings of the National Academy of Sciences of the United States of America - 26 Oct 1999
Gachotte D, Sen S E, Eckstein J, Barbuch R, Krieger M, Ray B D, Bard M
Abstract excerpt
The last unidentified gene encoding an enzyme involved in ergosterol biosynthesis in Saccharomyces cerevisiae has been cloned. This gene, designated ERG27, encodes the 3-keto sterol reductase, which, in concert with the C-4 sterol methyloxidase (ERG25) and the C-3 sterol dehydrogenase (ERG26), catalyzes the sequential removal of the two methyl groups at the sterol C-4 position. We developed a strategy to isolate...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Genes, Fungal
- Lanosterol
- Magnetic Resonance Spectroscopy
- Methylation
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae