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Article

Optimizing the APC gene mutation analysis in archival colorectal tumor tissue.

Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Mar 1999

Poncin J, Mulkens J, Arends J W, de Goeij A

Abstract excerpt

Critical steps in the methodology of mutation analysis on routinely fixed, paraffin-embedded samples have been evaluated, including extraction and purification of DNA, amplification of gene fragments in various sizes, and screening for mutations. The DNA was extracted from tissue sections with proteinase K, using various procedures, and purified. The mutation cluster region of the APC gene was amplified with...

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Identifiers

PMID: 10408788
DOI: 10.1097/00019606-199903000-00003

Genes

APC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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