Article
Simple, rapid, and accurate determination of deletion mutations by automated DNA sequencing of heteroduplex fragments of the adenomatous polyposis coli (APC) gene generated by PCR amplification.
Human mutation - 1 Jan 1993
Tamura K, Yamamoto Y, Saeki Y, Furuyama J, Utsunomiya J
Abstract excerpt
Germline mutations in patients with familial adenomatous polyposis were analyzed by polymerase chain reaction (PCR) amplification of the adenomatous polyposis coli gene. PCR products from heterozygous patients for deletions of this gene formed four distinct bands on polyacrylamide gel electrophor...
Topics
- Adenomatous Polyposis Coli
- Adult
- Base Sequence
- Child
- DNA
- Electrophoresis, Polyacrylamide Gel
- Female
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Pedigree
- Polymerase Chain Reaction
- Sequence Deletion