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Article

Rescue of dysfunctional deltaF508-CFTR chloride channel activity by IBMX.

The Journal of membrane biology - 1 Jul 1999

Schultz B D, Frizzell R A, Bridges R J

Abstract excerpt

Nucleotide-dependent gating of DeltaF508-CFTR was evaluated in membrane patches excised from HEK 293 and mouse L-cells and compared to observations on wt-CFTR channels recorded in the same expression systems. DeltaF508-CFTR exhibited PKA activated, ATP-dependent channel gating. When compared to wt-CFTR, the Km for ATP was increased by ninefold (260 micron vs. 28 micron) and maximal open probability (Po) was...

Topics

1-Methyl-3-isobutylxanthine
Adenosine Triphosphate
Animals
Cell Line
Chloride Channels
Cystic Fibrosis Transmembrane Conductance Regulator
Electric Conductivity
Humans
Ion Channel Gating
Mutation
Nucleotides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Patch-Clamp Techniques

Phosphodiesterase Inhibitors

Phosphorylation

Source

Identifiers

PMID: 10398760
DOI: 10.1007/s002329900537

Genes

CFTR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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