Article
On the mechanism of MgATP-dependent gating of CFTR Cl- channels.
The Journal of general physiology - 1 Jan 2003
Vergani Paola, Nairn Angus C, Gadsby David C
Abstract excerpt
CFTR, the product of the gene mutated in cystic fibrosis, is an ATPase that functions as a Cl(-) channel in which bursts of openings separate relatively long interburst closed times (tauib). Channel gating is controlled by phosphorylation and MgATP, but the underlying molecular mechanisms remain controversial. To investigate them, we expressed CFTR channels in Xenopus oocytes and examined, in excised patches, how...
Topics
- Adenosine Triphosphate
- Animals
- Binding Sites
- Cystic Fibrosis Transmembrane Conductance Regulator
- Dose-Response Relationship, Drug
- Female
- Humans
- Ion Channel Gating
- Mutation
- Xenopus laevis