Comparison of selectable and plaque assay systems to detect menadione- and UV-induced lacI mutations in mammalian cells.

doi:10.1016/s0027-5107(99)00094-9

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Comparison of selectable and plaque assay systems to detect menadione- and UV-induced lacI mutations in mammalian cells.

Mutation research - 30 Jun 1999

Andrew S E, Hsiao L, Milhausen K, Jirik F R

Abstract excerpt

We have compared the spontaneous mutation frequency and spectrum of lacI genes recovered from a rat embryonic fibroblast line transfected with a lambda-phage shuttle vector (Rat2lambdalacI) using both the traditional plaque assay as well as a positive selection assay. In addition, mutation frequencies and spectrum were determined after treatment of the cells with either the intracellular superoxide-generating...

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Comparison of selectable and plaque assay systems to detect menadione- and UV-induced lacI mutations in mammalian cells.

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